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Fast liquid chromatography coupled to electrospray tandem mass spectrometry peptide sequencing for cross‐species protein identification
Author(s) -
Witters Erwin,
Laukens Kris,
Deckers Peter,
Dongen Walter Van,
Esmans Eddy,
Onckelen Harry Van
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1173
Subject(s) - chemistry , chromatography , mass spectrometry , electrospray , quadrupole time of flight , tandem mass spectrometry , protein mass spectrometry , peptide , sample preparation in mass spectrometry , liquid chromatography–mass spectrometry , hybrid mass spectrometer , protein sequencing , selected reaction monitoring , analytical chemistry (journal) , peptide sequence , electrospray ionization , biochemistry , gene
Using a parallel microcolumn switching liquid chromatography set‐up coupled to a quadrupole time‐of‐flight mass spectrometer, a rapid liquid chromatography/mass spectrometric (LC/MS) protein identification method is presented. Without prior sample clean‐up up to 300 protein digest samples a day can be processed. Using data‐directed acquisition, up to 10 fragmentation analyses for each protein sample can be acquired in the same chromatographic run that can be used for database searching. Using internal peptide sequence information, protein databases and the various nucleic acid databases can both be queried for cross‐species identification of the protein sample. The method was evaluated and put into force to generate data for a tobacco cell culture protein database. Copyright © 2003 John Wiley & Sons, Ltd.