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Identification of plant sterols in hexaploid and tetraploid wheats using gas chromatography with mass spectrometry
Author(s) -
Pelillo Marco,
Iafelice Giovanna,
Marconi Emanuele,
Caboni Maria Fiorenza
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1156
Subject(s) - chemistry , sterol , mass spectrometry , threshing , food science , botany , chromatography , biology , biochemistry , cholesterol
Free sterols from hexaploid and tetraploid free‐threshing wheats ( Triticum aestivum L. and T. durum Desf.) and from their respective hulled wheats ( T. spelta L. and T. dicoccon Schrank) were analysed by gas chromatography with mass spectrometry. The qualitative analysis of sterols showed a similar pattern either between hexaploid ( T. aestivum , T. spelta ) and tetraploid ( T. durum , T. dicoccon ) wheats or between free‐threshing (T. aestivum , T. durum ) and hulled (T. spelta , T. dicoccon ) wheats. However, quantitative differences were found between tetraploid and hexaploid wheats, in that free sterol amounts in tetraploid wheats were 40% higher than in hexaploid ones. The mass spectra of the sterols were classified into four groups, taking into account the structural features of rings A and B. Typical mass spectral fragmentations of the four classes, and additional evidence related to the side chain of each molecule, were investigated together with their chromatographic behaviour, allowing identification of all the detected sterols. Copyright © 2003 John Wiley & Sons, Ltd.