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Sonic spray ionization applied to liquid chromatography/mass spectrometry analysis of endocrine‐disrupting chemicals in environmental water samples
Author(s) -
Benijts Tom,
Günther Wolfgang,
Lambert Willy,
Leenheer André De
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1131
Subject(s) - chemistry , atmospheric pressure chemical ionization , chromatography , mass spectrometry , chemical ionization , electrospray ionization , analytical chemistry (journal) , detection limit , direct electron ionization liquid chromatography–mass spectrometry interface , ambient ionization , ionization , desorption electrospray ionization , electrospray , high performance liquid chromatography , solvent , ion , organic chemistry
A sonic spray ionization liquid chromatography/mass spectrometry (LC/SSI‐MS) procedure combined with off‐line solid‐phase extraction was optimized for the analysis of 20 endocrine‐disrupting chemicals (EDCs) in water samples. Method development included a comparison of the novel sonic spray ionization (SSI) with more traditional ion sources, i.e. pneumatically assisted electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). It was demonstrated that SSI and ESI spectra were very similar, but were more prone to the formation of solvent cluster ions as compared with APCI spectra. This phenomenon was most prominent for SSI and resulted in an increased chemical background in full‐scan mass spectra. However, this chemical noise did not affect the overall sensitivity of SSI and ESI. After optimization of LC and MS parameters, the LC/SSI‐MS method was validated. Recoveries ranged from 76.3 up to 113.4% for all compounds. Limits of detection (LOD) and quantitation (LOQ) were established between 3.0 and 11.5 ng/L and 9.9 and 38.0 ng/L, respectively. Within‐day (n = 5) and between‐day (n = 5) reproducibility were investigated at three levels and ranged from 3.3–16.5% and 7.6–19.2%, respectively. Eight‐point calibration curves were established and showed linearity for all compounds (r 2 > 0.987) over a linear dynamic range of 10–10 000 ng/L. Copyright © 2003 John Wiley & Sons, Ltd.