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Improved peptide sequencing using isotope information inherent in tandem mass spectra
Author(s) -
Can William R.,
Jarman Kenneth D.
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1119
Subject(s) - chemistry , tandem , tandem mass spectrometry , peptide , isotope , mass spectrometry , mass spectrum , ion , chromatography , analytical chemistry (journal) , biochemistry , organic chemistry , materials science , physics , quantum mechanics , composite material
We demonstrate here the use of natural isotopic ‘labels’ in peptides to aid in the identification of peptides with a de novo algorithm. Using data from ion trap tandem mass spectrometric (MS/MS) analysis of 102 tryptic peptides, we have analyzed multiple series of peaks within LCQ MS/MS spectra that ‘spell’ peptide sequences. Isotopic peaks from naturally abundant isotopes are particularly prominent even after peak centroiding on y‐ and b‐series ions and lead to increased confidence in the identification of the precursor peptides. Sequence analysis of the MS/MS data is accomplished by finding sequences and subsequences in a hierarchical manner within the spectra. Copyright © 2003 John Wiley & Sons, Ltd.