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Electrospray ionization from a droplet deposited on a surface‐modified glass rod
Author(s) -
Jeng Jingyueh,
Shiea Jentaie
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1109
Subject(s) - chemistry , electrospray ionization , aqueous solution , fiber , electrospray , analytical chemistry (journal) , mass spectrometry , optical fiber , wetting , ionization , ion , quadrupole mass analyzer , chromatography , optics , composite material , materials science , organic chemistry , physics
This paper reports development of a non‐mechanical electrospray ionization (ESI) method to generate electrospray from a droplet deposited on an optical fiber coated with a thin gold or Nafion film. Modification of the surface of the optical fiber in this manner increases its wettability, such that a droplet of the aqueous sample solution can adhere sufficiently strongly to the tip of the fiber. The aqueous sample solution was deposited near the tip of the fiber with a micropipette. When a high voltage (2000 V) was applied to the fiber by electrical connection through the gold film, the sample solution moved and hung at the tip of the fiber. Simultaneously, ESI was generated from the sample droplet. Multiply charged peptide and protein ions were detected by connecting the ESI source to a quadrupole mass analyzer. Copyright © 2003 John Wiley & Sons, Ltd.