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Parallel high‐throughput accurate mass measurement using a nine‐channel multiplexed electrospray liquid chromatography ultraviolet time‐of‐flight mass spectrometry system
Author(s) -
Fang Liling,
Demee Michael,
Cournoyer Jason,
Sierra Teresa,
Young Connie,
Yan Bing
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1065
Subject(s) - chemistry , mass spectrometry , chromatography , time of flight mass spectrometry , throughput , electrospray , analytical chemistry (journal) , multiplexing , ultraviolet , optoelectronics , computer science , organic chemistry , ion , telecommunications , physics , wireless , ionization
A nine‐channel multiplexed electrospray (MUX) liquid chromatography ultraviolet time‐of‐flight mass spectrometry (LC/UV/TOFMS) system has been used to simultaneously measure accurate masses of eluting components from eight parallel gradient LC columns. Accuracies better than 5 and 10 ppm were achieved for 50 and 80% of samples, respectively, from a single batch analysis of ten plates (960 samples) of a Fmoc‐Asp(OtBu)‐OH and reserpine mixture. Combinatorial library compounds were analyzed using this parallel high‐throughput system in both positive and negative modes to rigorously verify expected products and identify side products. A mass accuracy of 10 ppm root mean square (RMS) is routinely obtained for combinatorial library samples from this high‐throughput accurate mass LC/MS system followed by automated data processing. This mass accuracy is critical in revealing combinatorial synthesis problems that would be missed by unit mass measurement. Copyright © 2003 John Wiley & Sons, Ltd.