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Flow injection of the lock mass standard for accurate mass measurement in electrospray ionization time‐of‐flight mass spectrometry coupled with liquid chromatography
Author(s) -
Charles Laurence
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1060
Subject(s) - chemistry , mass spectrometry , chromatography , analyte , electrospray ionization , analytical chemistry (journal) , direct electron ionization liquid chromatography–mass spectrometry interface , ionization , electrospray , sample preparation in mass spectrometry , ion source , time of flight mass spectrometry , extractive electrospray ionization , ion , chemical ionization , organic chemistry
A method of flow injection of the lock mass for accurate mass measurement using electrospray ionization time‐of‐flight mass spectrometry is described. The reference compound is introduced in the chromatographic effluent via a six‐port valve placed post‐column, prior to the split connector. Flow injection is performed in such a way that the reference elution peak is superimposed in the total ion current and partially overlaps that of the investigated analyte, allowing independent ionization of the two compounds and thus accurate mass measurement with no ion suppression effects. Different lock mass molecules can be injected in a single analytical run to target various analytes. The performance of this methodology is demonstrated in both isocratic and gradient liquid chromatography modes. The molecular ion of the flow‐injected lock mass could also be used as a reference for mass measurement of the in‐source fragments of the analytes. Good mass accuracy, within 4 mDa of the theoretical values, was obtained. Copyright © 2003 John Wiley & Sons, Ltd.