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Sequencing of bacitracin A and related minor components by liquid chromatography/electrospray ionization ion trap tandem mass spectrometry
Author(s) -
Govaerts Cindy,
Li Chunlan,
Orwa Jennifer,
Van Schepdael Ann,
Adams Erwin,
Roets Eugène,
Hoogmartens Jos
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1058
Subject(s) - chemistry , chromatography , electrospray ionization , bacitracin , mass spectrometry , ion trap , electrospray , tandem mass spectrometry , top down proteomics , liquid chromatography–mass spectrometry , protein mass spectrometry , analytical chemistry (journal) , antibiotics , biochemistry
A selective reversed‐phase liquid chromatography/mass spectrometry (LC/MS n ) method is described for the characterization of related compounds in commercial bacitracin samples. Mass spectral data for these polypeptide antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive and negative ion mode. The LCQ ion trap is ideally suited for the sequencing of those linear side‐chain cyclized peptides because it provides on‐line LC/MS n capability. Using this method bacitracin A, 1‐epibacitracin A, bacitracins B 1 , B 2 , B 3 and bacitracin F were sequenced and previous sequencing was confirmed. Bacitracins C 1 , C 2 , C 3 , D, H 2 and H 3 were resolved chromatographically and their ring portion was sequenced for the first time. Four components not described in the literature (1‐epibacitracin B 1 , 1‐epibacitracin B 2 , 1‐epibacitracin C 1 and H 4 ) were sequenced completely for the first time. The main advantage of this hyphenated LC/MS n technique is the characterization of the related substances without time‐consuming isolation and purification procedures. Copyright © 2003 John Wiley & Sons, Ltd.