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Simultaneous quantitation of enalapril and enalaprilat in human plasma by 96‐well solid‐phase extraction and liquid chromatography/tandem mass spectrometry
Author(s) -
Lee Jaeick,
Son Junghyun,
Lee Mijin,
Lee Kyung Tae,
Kim DongHyun
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1040
Subject(s) - enalaprilat , chemistry , chromatography , enalapril , solid phase extraction , tandem mass spectrometry , selected reaction monitoring , liquid chromatography–mass spectrometry , mass spectrometry , analyte , extraction (chemistry) , calibration curve , human plasma , detection limit , angiotensin converting enzyme , medicine , blood pressure , radiology
A sensitive and rapid method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) combined with rapid solid‐phase extraction (SPE) has been developed and validated for the quantitative determination of enalapril and its active metabolite enalaprilat in human plasma. After addition of internal standard to human plasma, samples were extracted by 96‐well SPE cartridge. The extracts were analyzed by HPLC with the detection of the analyte in the multiple reaction monitoring (MRM) mode. This method for the simultaneous determination of enalapril and enalaprilat was accurate and reproducible, with respective limits of quantitation of 0.2 and 1.0 ng/mL in plasma. The standard calibration curves for both enalapril and enalaprilat were linear (r 2 = 0.9978 and 0.9998) over the concentration ranges 0.2–200 and 1.0–100 ng/mL in human plasma, respectively. The intra‐ and inter‐day precision over the concentration range for enalapril and enalaprilat were lower than 13.3 and 15.4% (relative standard deviation, %RSD), and accuracy was between 89.2–105.0 and 91.9–104.7%, respectively. Copyright © 2003 John Wiley & Sons, Ltd.