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Analysis of urinary nucleosides. III. Identification of 5′‐deoxycytidine in urine of a patient with head and neck cancer
Author(s) -
Dudley Edward,
Lemiere Filip,
Van Dongen Walter,
Langridge James I.,
ElSharkawi Salah,
Games David E.,
Esmans Eddy L.,
Newton Russell P.
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1034
Subject(s) - chemistry , urine , chromatography , mass spectrometry , deoxycytidine , nucleoside , high performance liquid chromatography , protonation , urinary system , cancer , stereochemistry , medicine , biochemistry , ion , organic chemistry , gemcitabine
Modified nucleosides in the urine have been postulated to be diagnostic indices of disease, particularly of cancer. The urine of a patient with terminal head and neck cancer has been found to contain a modified nucleoside with a protonated molecule of m/z 228. By means of high‐performance liquid chromatography/ion trap mass spectrometry (HPLC/ITMS) and capillary liquid chromatography/triple quadruple mass spectrometry (CapLC/TQMS) we have identified the compound as 5′‐deoxycytidine. This is the first report of 5′‐deoxycytidine in man: in addition to the elucidation of its structure, its possible origins and the potential significance of its occurrence are discussed. Copyright © 2003 John Wiley & Sons, Ltd.