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Identification of caffeic acid derivatives in Actea racemosa ( Cimicifuga racemosa , black cohosh) by liquid chromatography/tandem mass spectrometry
Author(s) -
Li Wenkui,
Sun Yongkai,
Liang Wenzhong,
Fitzloff John F.,
van Breemen Richard B.
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1008
Subject(s) - caffeic acid , chemistry , black cohosh , ferulic acid , chromatography , tandem mass spectrometry , electrospray ionization , mass spectrometry , organic chemistry , antioxidant , menopause , biology , genetics
Caffeic acid derivatives occurring in black cohosh [ Cimicifuga racemosa (L.) Nutt., Actaea racemosa (Ranunculaceae)], some of which may have pharmacological activity, were analyzed using high‐performance liquid chromatography (HPLC) electrospray ionization tandem mass spectrometry (ESI‐MS/MS) with the aim of developing a methodology for their rapid identification in a complex plant matrix. Based on these studies, structurally characteristic product ions and neutral molecule losses were identified, which were then used during LC/MS/MS with product ion scanning, precursor scanning and constant neutral loss scanning to detect caffeic acid derivatives in a crude extract of black cohosh. Several caffeic acid derivatives were detected, and the identification of six of them were confirmed by comparison with authentic standards including caffeic acid, ferulic acid, isoferulic acid, fukinolic acid, cimicifugic acid A, and cimicifugic acid B. Four other compounds were detected that appeared to be caffeic acid derivatives based on LC/MS/MS retention times, molecular weights, and fragmentation patterns during MS/MS. Since standards were unavailable for these four compounds, they were tentatively identified using LC/MS/MS as cimicifugic acid E, cimicifugic acid F, dehydrocimicifugic acid A, and dehydrocimicifugic acid B. Dehydrocimicifugic acid A and dehydrocimicifugic acid B have not been reported previously to be constituents of black cohosh. Copyright © 2003 John Wiley & Sons, Ltd.