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Derivatisation for the characterisation of neutral oxosteroids by electrospray and matrix‐assisted laser desorption/ionisation tandem mass spectrometry: the Girard P derivative
Author(s) -
Griffiths William J.,
Liu Suya,
Alvelius Gunvor,
Sjövall Jan
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1002
Subject(s) - chemistry , mass spectrometry , analyte , chromatography , protein mass spectrometry , tandem mass spectrometry , sample preparation in mass spectrometry , electrospray , reagent , matrix assisted laser desorption/ionization , steroid , matrix (chemical analysis) , electrospray ionization , analytical chemistry (journal) , desorption , organic chemistry , biochemistry , hormone , adsorption
Abstract The identification, quantification and localisation of steroids in biological fluids and tissues are subjects of considerable importance. Not only do steroids have classical hormonal properties via binding to nuclear receptors, they can also elicit cellular responses via interactions with other proteins. For mass spectrometric analysis, neutral steroids are not readily ionised by either electrospray (ES) or matrix‐assisted laser desorption/ionisation (MALDI). In this communication a derivatisation protocol is presented which allows for the rapid analysis of neutral oxosteroids by both ES and MALDI mass spectrometry. Neutral oxosteroids are derivatised to Girard P hydrazones. When analysed by tandem mass spectrometry the derivatised steroids fragment to give structurally informative spectra allowing subsequent steroid identification. The derivatisation method is simple, the reagents are commercially available, and reaction products are easily isolated from the reaction mixture. Analyte identification can be performed at the sub‐pg level. Copyright © 2003 John Wiley & Sons, Ltd.