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Direct tandem mass spectrometric analysis of amino acids in dried blood spots without chemical derivatization for neonatal screening
Author(s) -
Nagy Kornél,
Takáts Zoltán,
Pollreisz Ferenc,
Szabó Teréz,
Vékey Károly
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1000
Subject(s) - chemistry , derivatization , tandem mass spectrometry , tandem , chromatography , calibration curve , selected reaction monitoring , electrospray , dried blood , detection limit , spots , mass spectrometry , analytical chemistry (journal) , materials science , composite material
Neonatal screening performed by electrospray tandem mass spectrometry is a powerful technique in clinical diagnostics. In the present paper an alternative to the widely accepted method involving butylation has been developed. In the new method butylation is not required, and multiple reaction monitoring (MRM) was used instead of constant neutral loss scanning. The method was optimized for detection of 23 L‐amino acids in their native form. Quantitation was based on isotope‐labeled internal standards, calibration curves were linear from 0 to 500 μmol/L, and detection limits were in the range 2–42 μmol/L. The utility of the present technique is illustrated in the case of one neonate suffering from citrullinaemia. Copyright © 2003 John Wiley & Sons, Ltd.