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Estimation of binding sites from the adsorption profile of complexed DNA
Author(s) -
Ghosh Subhendu,
Mookerjee Anjali
Publication year - 1981
Publication title -
international journal of quantum chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.484
H-Index - 105
eISSN - 1097-461X
pISSN - 0020-7608
DOI - 10.1002/qua.560200118
Subject(s) - adsorption , intercalation (chemistry) , dna , chemistry , langmuir , scatchard plot , langmuir adsorption model , binding site , electrostatic interaction , inorganic chemistry , biochemistry , chemical physics
The adsorption profiles of free and Daunomycin bound DNA at an alumina–H 2 O interface have been studied. Proper experimental conditions were maintained to considerably reduce intercalation and to make electrostatic binding predominant in the DNA–Daunomycin complex. For both free and complexed DNA, Langmuir plots gave straight lines. Adsorption of drug bound DNA was less than that of free DNA. This has been explained from the viewpoint of electrostatic interactions between adsorbate and adsorbent. A Langmuir‐type model for adsorption of polymers combined with Scatchard equation have been used to estimate the average value of percent of phosphates in DNA stacked with Daunomycin.