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Effect of insulin on intracellular ph as observed by 31 p NMR spectroscopy
Author(s) -
Moore Richard D.,
Gupta Raj K.
Publication year - 2009
Publication title -
international journal of quantum chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.484
H-Index - 105
eISSN - 1097-461X
pISSN - 0020-7608
DOI - 10.1002/qua.560180708
Subject(s) - intracellular , intracellular ph , phosphofructokinase , phosphocreatine , chemistry , nuclear magnetic resonance spectroscopy , glycolysis , intracellular fluid , pi , insulin , limiting , biophysics , nuclear magnetic resonance , biochemistry , enzyme , stereochemistry , biology , endocrinology , energy metabolism , mechanical engineering , physics , engineering
31 P nuclear magnetic resonance (NMR) is used to determine the effect of insulin upon intracellular pH in frog skeletal muscle. An equation is derived which converts the difference between the phosphocreatine peak and the P i peak in the NMR spectra into an estimate of intracellular pH. Insulin produces a statistically significant increase in intracellular pH, as determined by this noninvasive technique, by an amount sufficient to activate phosphofructokinase, the key rate‐limiting enzyme of glycolysis.