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Effectiveness of barrier film with a cellulose coating that carries nisin blends for the inhibition of listeria monocytogenes
Author(s) -
Matthews Brian,
Mangalasary Sunil,
Darby Duncan,
Cooksey Kay
Publication year - 2010
Publication title -
packaging technology and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 50
eISSN - 1099-1522
pISSN - 0894-3214
DOI - 10.1002/pts.894
Subject(s) - nisin , listeria monocytogenes , antimicrobial , food science , agar , chemistry , population , microbiology and biotechnology , cellulose , biology , bacteria , medicine , biochemistry , genetics , environmental health
The objective of this study was to determine the effectiveness of two nisin blend antimicrobial agents (Guardian NR 250, Novagard CBI) incorporated into a cellulose coating that was applied onto a barrier film against Listeria monocytogenes . The minimum inhibitory concentration (MIC) of the agents in solution against L. monocytogenes was found to be 2.74 mg/ml for the two nisin blends. The concentrations tested for both nisin blend treatments were 5.49, 10.9, 16.4 and 21.9 mg/ml. Guardian NR 250 resulted in wider zones of inhibition compared to the Novagard CBI at all levels tested. The MIC for Guardian NR 250 in the film was 5.49 mg/ml. Films containing Novagard CBI did not show any antimicrobial activity. A food challenge study was conducted using the film containing Guardian NR 250 at levels of 5.49 and 21.9 mg/ml. Inoculated fresh beef cubes were individually packaged with pre‐made barrier film pouches that had an interior cellulose coating containing the antimicrobial agent and stored at 4°C for 36 days. Bacterial colonies were enumerated every 6 days on modified Oxford agar. There was no significant difference in the L. monocytogenes population between two levels of Guardian NR 250 throughout the study. There was statistically significant inhibition of L. monocytogenes for both levels of Guardian NR 250 during 18–30 days of storage compared to a control film without the antimicrobial agent. Copyright © 2010 John Wiley & Sons, Ltd.