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Preservation of sliced ham through triclosan active film
Author(s) -
Camilloto Geany Peruch,
de Fátima Ferreira Soares Nilda,
dos Santos Pires Ana Clarissa,
de Paula Filipe Santos
Publication year - 2009
Publication title -
packaging technology and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 50
eISSN - 1099-1522
pISSN - 0894-3214
DOI - 10.1002/pts.871
Subject(s) - antimicrobial , staphylococcus aureus , triclosan , food science , microbiology and biotechnology , agar , active packaging , food packaging , agar diffusion test , ultimate tensile strength , escherichia coli , chemistry , materials science , biology , bacteria , composite material , medicine , biochemistry , genetics , pathology , gene
Active packaging is an alternative to preserve perishable food. In this work, polyethylene antimicrobial active films containing different levels of triclosan (0, 2000 and 4000 mg kg −1 ) were developed by extrusion. The films' efficacies were evaluated against Escherichia coli, Staphylococcus aureus, Listeria innocua, Salmonella choleraesuis and Pseudomonas aeruginosa growth using agar diffusion test and by monitoring the inhibition of E. coli and S. aureus inoculated on sliced cooked ham. The mechanical characteristics of the films were also evaluated with Universal Test Machine (Instron). The incorporation of triclosan did not affect the mechanical properties of antimicrobial films compared to the control film. The average film thickness was 82.0 µ m and the tensile strength and elongation to break were 30.3 N and 46.2%, respectively. Films containing triclosan showed an antimicrobial effect in vitro against E. coli and S. aureus , with formation of an inhibition halo for both. However, this result was not observed for L. innocua, S. choleraesuis and P. aeruginosa , although, a decrease in colony density occurred around the film for both incubation temperatures (7 ± 2°C and 35 ± 2°C). Sliced ham packed with the antimicrobial films showed a reduction of 1.5 logarithmic cycles in comparison to ham in contact with a control film after 12 days of storage at 7 ± 2°C, for E. coli and S. aureus. Antimicrobial films present potential for application as active packaging materials, as they showed effective against some pathogenic microorganisms that can be transmitted by foods. Copyright © 2009 John Wiley & Sons, Ltd.