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Bioactive phenolic derivatives from Acaena splendens methanol extract
Author(s) -
Backhouse N.,
Delporte C.,
Negrete R.,
Feliciano S. A. San,
LópezPérez J. L.
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.997
Subject(s) - traditional medicine , pharmacognosy , methanol , chemistry , folk medicine , stereochemistry , biology , biological activity , medicine , organic chemistry , in vitro , biochemistry
Acaena splendens H. et A. has been used in Chilean folk medicine for the treatment of fever and inflammation. A description of the in vivo reduction of bacterial pyrogen‐induced fever in rabbits and carrageenan‐induced paw oedema in guinea pigs is presented. The methanol extract named ME‐1, obtained after succesive extractions with petroleum ether and dichloromethane, showed a strong antipyretic action (45.7% of effect), though the antiinflammatory activity was only observed after submitting this extract to column fractionation, giving a crude mixture of flavonoids named C4 with both activities (55.7% and 98.9% of antiinflammatory and antipyretic effect respectively at a dose of 600 mg/kg). The bioassay‐guided fractionation by column chromatography afforded the active fraction, which contained (‐,‐)‐epicatechin, tiliroside, 7‐O‐acetyl‐3‐O‐β‐ D ‐glucosyl‐kaempferol and 7‐β‐ D ‐glucosyloxy‐5‐hydroxy‐chromone. Copyright © 2002 John Wiley & Sons, Ltd.