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Effect of quercetin on plasma extravasation in rat CNS and dura mater by ACE and NEP inhibition
Author(s) -
Cyrino Luiz A. R.,
Cardoso Ronie C. F.,
Hackl Luciane P. N.,
Nicolau Mauro
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.987
Subject(s) - dura mater , extravasation , pia mater , evans blue , phosphoramidon , olfactory bulb , quercetin , cerebellum , neprilysin , substance p , chemistry , endocrinology , captopril , medicine , central nervous system , anatomy , biology , receptor , pathology , biochemistry , enzyme , neuropeptide , antioxidant , blood pressure
Abstract The effects of quercetin on substance P‐induced plasma protein extravasation (PE) in the rat dura mater, cerebellum, olfactory bulb and cortex and also its modulation by endopeptidases, angiotensin‐converting enzyme (ACE) and neutral endopeptidase (NEP) were studied. PE was assessed by photometric measurement of extravasated Evans blue. Substance P (SP) and NEP or ACE inhibitors increased the PE in dura mater. Pretreatment with captopril or phosphoramidon potentiated PE induced by SP in the dura mater and cerebellum, respectively. Quercetin increased the PE in the dura mater, cerebellum and cortex. Further results suggested that the PE induced by SP in the dura mater was enhanced by pretreatment with quercetin, similar to that observed with selective peptidase inhibitors. Quercetin‐stimulated extravasation in all tissues was abolished by NK‐1 receptor blockade. These results suggest that quercetin increases PE in the dura mater and CNS tissues by inhibiting NEP and/or ACE, showing that the effect induced in the dura mater, cerebellum and cortex occurs through endogenous SP accumulation. Copyright © 2002 John Wiley & Sons, Ltd.