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Effect of Mangifera indica L. extract (QF808) on protein and hepatic microsome peroxidation
Author(s) -
Martínez Gregorio,
Giuliani Attilia,
León Olga Sonia,
Pérez Gema,
Núñez Selles Alberto J
Publication year - 2001
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.980
Subject(s) - mangiferin , lipid peroxidation , microsome , chemistry , ascorbic acid , antioxidant , biochemistry , microsoma , bovine serum albumin , nicotinamide adenine dinucleotide phosphate , mangifera , oxidase test , enzyme , chromatography , biology , food science , horticulture
The antioxidant activities of QF808, a steam bark extract of Mangifera indica L., were studied on hydroxyl‐mediated oxidation of bovine serum albumin (BSA) and in a hepatic microsome system. The extract was effective in reducing the oxidation of BSA, since its half‐ maximal inhibition concentration (IC 50 ) was 0.0049% w/v in the inhibition of carbonyl group formation and lower than 0.0025% w/v in the inhibition of sulfhydryl group loss. QF808 inhibited lipid peroxidation which was initiated enzymatically by reduced nicotinamide adenine dinucleotide phosphate (NADPH), IC 50 = 0.00075% w/v, or non‐enzymatically by ascorbic acid, IC 50 = 0.0126% w/v. The extract tested did not inhibit NADPH‐dependent cytochrome P‐450 reductase activity, since it had no effect on the oxidation rate of NADPH. These results suggest that QF808 has an antioxidant activity, probably due to its ability to scavenge free radicals involved in microsome lipid peroxidation. In addition, QF808 antioxidant profile in vitro is probably similar to its principal polyphenolic component, mangiferin, a glycosylated xanthone. Copyright © 2001 John Wiley & Sons, Ltd.