Premium
Effects of tanshinone I isolated from Salvia miltiorrhiza Bunge on arachidonic acid metabolism and in vivo inflammatory responses
Author(s) -
Kim Sung Young,
Moon Tae Cheol,
Chang Hyeun Wook,
Son Kun Ho,
Kang Sam Sik,
Kim Hyun Pyo
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.941
Subject(s) - salvia miltiorrhiza , arachidonic acid , in vivo , pharmacology , chemistry , arachidonate 5 lipoxygenase , lipoxygenase , cyclooxygenase , biological activity , phospholipase a2 , biochemistry , in vitro , biology , enzyme , medicine , alternative medicine , microbiology and biotechnology , pathology , traditional chinese medicine
Arachidonic acid (AA) mainly released from the cell membrane by phospholipase A 2 (PLA 2 ) is converted to eicosanoids by the action of cyclooxygenase (COX) and lipoxygenase (LO). In order to find the specific inhibitors of AA metabolism especially PLA 2 and COX‐2, 300 plant extracts were evaluated for their inhibitory activity on PGD 2 production from cytokine‐induced mouse bone marrow‐derived mast cells in vitro . From this screening procedure, the methanol extract of Salvia miltiorrhiza was found to inhibit PGD 2 production and the ethyl acetate subfraction gave the strongest inhibition of five subfractions tested. From this ethyl acetate subfraction, an activity‐guided isolation finally gave tanshinone I as an active principle. This investigation deals with the effects of tanshinone I on AA metabolism from lipopolysaccharide (LPS)‐induced RAW 264.7 cells and in vivo antiinflammatory activity. Tanshinone I inhibited PGE 2 formation from LPS‐induced RAW macrophages (IC 50 = 38 μ M ). However, this compound did not affect COX‐2 activity or COX‐2 expression. Tanshinone I was found to be an inhibitor of type IIA human recombinant sPLA 2 (IC 50 = 11 μ M ) and rabbit recombinant cPLA 2 (IC 50 = 82 μ M ). In addition, tanshinone I showed in vivo antiinflammatory activity in rat carrageenan‐induced paw oedema and adjuvant‐induced arthritis. Copyright © 2002 John Wiley & Sons, Ltd.