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Inhibition of lipid peroxidation and oxidative DNA damage by Ganoderma lucidum
Author(s) -
Lee JongMin,
Kwon Hoonjeong,
Jeong Hoon,
Lee June Woo,
Lee Seung Yong,
Baek Seong Jin,
Surh YoungJoon
Publication year - 2001
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.830
Subject(s) - superoxide , reactive oxygen species , chemistry , lipid peroxidation , biochemistry , dna damage , antioxidant , hydroxyl radical , pro oxidant , hydrogen peroxide , radical , oxidative phosphorylation , oxidative stress , dna , enzyme
Reactive oxygen species (ROS), such as superoxide anions and hydroxyl radicals, are associated with carcinogenesis and other pathophysiological conditions. Therefore, elimination or inactivation of ROS or inhibition of their excess generation may be beneficial in terms of reducing the risk for cancer and other diseases. Ganoderma lucidum has been used in traditional oriental medicine and has potential antiinflammatory and antioxidant activities. In the present study, we tested the amino‐polysaccharide fraction (designated as ‘G009’) from Ganoderma lucidum for the ability to protect against oxidative damage induced by ROS. G009 significantly inhibited iron‐induced lipid peroxidation in rat brain homogenates and showed a dose‐dependent inactivation of hydroxyl radicals and superoxide anions. It also reduced strand breakage in ϕX174 supercoiled DNA caused by UV‐induced photolysis of hydrogen peroxide and attenuated phorbol ester‐induced generation of superoxide anions in differentiated human promyelocytic leukaemia (HL‐60) cells. These findings suggest that G009 from Ganoderma lucidum possesses chemopreventive potential. Copyright © 2001 John Wiley & Sons, Ltd.