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Wound healing activity of acylated iridoid glycosides from Scrophularia nodosa
Author(s) -
Stevenson Philip C.,
Simmonds Monique S. J.,
Sampson Julia,
Houghton Peter J.,
Grice Peter
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.798
Subject(s) - catalpol , iridoid , glycoside , traditional medicine , pharmacognosy , chemistry , stereochemistry , in vitro , botany , biological activity , biology , biochemistry , medicine
Three acylated iridoid glycosides ( E )‐6‐ O ‐(2″, 4″‐diacetyl‐3″ ‐ O‐p ‐methoxycinnamoyl)‐α‐ L ‐rhamnopyranosyl catalpol (scopolioside A) ( 1 ), ( E )‐6‐ O ‐(2″‐acetyl‐3″, 4″‐di‐ O,O‐p ‐methoxycinnamoyl)‐α‐ L ‐rhamnopyranosyl catalpol (scrophuloside A 4 ) ( 2 ) and ( E )‐6‐ O ‐(2″,3″‐diacetyl‐4″‐ O‐p ‐methoxycinnamoyl)‐α‐ L ‐rhamnopyranosyl catalpol (scrovalentinoside) ( 3 ) have been isolated from the dried seed pods of Scrophularia nodosa by HPLC. Their structures were determined by 1D and 2D NMR, UV/Vis and mass spectroscopy and by comparison with published data. All three compounds were shown in vitro to stimulate the growth of human dermal fibroblasts. The effect was negatively dose‐dependent for 2 and 3 for which fibroblast growth stimulation was highest at 0.78 μg/mL but was not significantly different from the control at 100 μg/mL. The presence of these compounds in the mature seed pods may explain the ethnobotanical use of this plant in Europe for healing wounds. Copyright © 2002 John Wiley & Sons, Ltd.