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Inhibition of tumour necrosis factor‐α secretion from rat astrocytes by Sesim‐Tang
Author(s) -
Kim H. M.,
Shin H. Y.,
Lee S. P.,
Ryu S. T.,
Shin T. Y.,
Chae H. J.,
Kim H. R.,
Lyu Y. S.,
Lim K. S.
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.787
Subject(s) - secretion , tumor necrosis factor alpha , lipopolysaccharide , astrocyte , endocrinology , medicine , biology , interleukin 6 , interleukin , cytokine , neuroglia , alpha (finance) , central nervous system , construct validity , nursing , patient satisfaction
Substance P (SP) can stimulate secretion of tumour necrosis factor‐α (TNF‐α) from astrocytes stimulated with lipopolysaccharide (LPS). In this study, we have examined whether an aqueous extract of Sesim‐Tang inhibits the secretion of TNF‐α from primary cultures of rat astrocytes. Sesim‐Tang (10–1000μg/mL) significantly inhibited the TNF‐α secretion by astrocytes stimulated with LPS and SP. Interleukin‐1 (IL‐1) has been shown to elevate TNF‐α secretion from LPS‐stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore examined whether IL‐1 mediated inhibition of TNF‐α secretion from primary astrocytes by Sesim‐Tang. Treatment with Sesim‐Tang (10–1000 μg/mL) of astrocytes stimulated with both LPS and SP decreased IL‐1 secretion significantly. Moreover, the secretion of TNF‐α by LPS and SP in astrocytes was progressively inhibited with increasing amounts of IL‐1 neutralizing antibody. Our results suggest that Sesim‐Tang may inhibit TNF‐α secretion by inhibiting IL‐1 secretion and that Sesim‐Tang has an antiinflammatory activity in the central nervous system. Copyright © 2002 John Wiley & Sons, Ltd.