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Hepatoprotective activity of indigtone—a bioactive fraction from Indigofera tinctoria Linn.
Author(s) -
Singh B.,
Saxena A. K.,
Chandan B. K.,
Bhardwaj V.,
Gupta V. N.,
Suri O. P.,
Handa S. S.
Publication year - 2001
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.760
Subject(s) - hepatotoxin , traditional medicine , carbon tetrachloride , chemistry , liver injury , fractionation , pharmacology , petroleum ether , ccl4 , bilirubin , phytotherapy , toxicity , biology , medicine , chromatography , pathology , organic chemistry , alternative medicine , extraction (chemistry)
A bioactive fraction, indigtone (FA), obtained by fractionation of a petroleum ether extract of the aerial parts of Indigofera tinctoria , showed significant dose related hepatoprotective activity against CCl 4 induced liver injury in rats and mice. Hexobarbitone induced ‘sleeptime’, zoxazolamine induced ‘paralysis time’, levels of transaminases, bilirubin and total protein in serum were employed as indices of liver injury. Pre and post treatment with FA significantly reversed the majority of the parameters altered by the hepatotoxin. This indicated the preventive and restorative effect of FA in the process of CCl 4 induced liver damage. The fraction possessed a high therapeutic ratio, as no mortality was observed up to a dose of 2 g/kg p.o. in mice. Copyright © 2001 John Wiley & Sons, Ltd.