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Inhibitory effects of Keishi‐bukuryo‐gan on free radical induced lysis of rat red blood cells
Author(s) -
Sekiya Nobuyasu,
Goto Hirozo,
Shimada Yutaka,
Terasawa Katsutoshi
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.757
Subject(s) - haemolysis , antioxidant , red blood cell , polyphenol , ingestion , hemolysis , chemistry , lysis , pharmacology , biochemistry , medicine , immunology
Keishi‐bukuryo‐gan (KBG) prevents the progression of atherosclerosis in cholesterol‐fed rabbits by its antioxidative effect. The present study was to test our hypothesis that ingestion of KBG would protect red blood cell (RBC) membranes from free radical induced oxidation if polyphenolic antioxidants in KBG could be absorbed and circulated in the blood. When incubated with a RBC suspension, KBG and four of five herb medicines constituting KBG provided strong protection for RBC membranes against haemolysis induced by 2,2‐azo‐bis (2‐amidinopropane) dihydrochloride (AAPH), an azo free radical initiator. The inhibitory effect was dose dependent at concentrations of 100–1000 μg/mL. Furthermore, the ingestion of 200 mg of KBG was associated with a significant decrease in susceptibility of RBC to haemolysis in rats. Copyright © 2002 John Wiley & Sons, Ltd.