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Inhibitory effects of Korean plants on HIV‐1 activities
Author(s) -
Min Byung Sun,
Kim Young Ho,
Tomiyama Miyuki,
Nakamura Norio,
Miyashiro Hirotsugu,
Otake Toru,
Hattori Masao
Publication year - 2001
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.751
Subject(s) - biology , traditional medicine , reverse transcriptase , protease , pharmacognosy , biological activity , botany , enzyme , biochemistry , in vitro , rna , medicine , gene
In the search for novel anti‐human immunodeficiency virus type 1 (anti‐HIV‐1) agents from natural sources, 49 MeOH extracts of Korean plants were screened for their inhibitory effects against RNA‐dependent DNA polymerase (RT) and ribonuclease H (RNase H) activities of HIV‐1 reverse transcriptase and HIV‐1 protease, and anti‐HIV‐1 activity. Regarding the HIV‐1 reverse transcriptase, Agrimonia pilosa (whole plant), Cornus kousa (stem and leaf), Limonium tetragonum (root) and Mallotus japonicus (stem) showed significant inhibitory activity on RT activity with 50% inhibitory activity (IC 50 ) of 8.9, 6.3, 7.5 and 11.9 µg/mL, respectively, whereas Agrimonia pilosa was also active against RNase H activity (IC 50 = 98.4 µg/mL). Four plants, namely Agrimonia pilosa (whole plant), Atractylodes japonica (root), Clematis heracleifolia (whole plant) and Syneilesis palmata (whole plant), were appreciably active (<35%) against recombinant HIV‐1 protease at a concentration of 100 µg/mL. Crinum asiaticum var. japonicum (root) showed significant anti‐HIV‐1 activity (ED 50 = 12.5 µg/mL) with a favourable SI value of 16. Copyright © 2001 John Wiley & Sons, Ltd.