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Cytotoxic activity of Typhonium flagelliforme (Araceae)
Author(s) -
Choo CheeYan,
Chan KitLam,
Takeya Koichi,
Itokawa Hideji
Publication year - 2001
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.717
Subject(s) - araceae , traditional medicine , chemistry , cytotoxic t cell , mtt assay , pharmacognosy , chloroform , food science , biology , botany , chromatography , biochemistry , medicine , biological activity , in vitro
The plant Typhonium flagelliforme (Araceae), commonly known as the ‘rodent tuber’, is often included as an essential ingredient in various herbal remedies recommended for cancer therapies in Malaysia. Various extracts prepared from either the roots, tubers, stems or leaves were tested for cytotoxic activity on murine P388 leukaemia cells using the MTT assay method. Both the chloroform (IC 50 = 6.0 µg/mL) and hexane (IC 50 = 15.0 µg/mL) extract from the ‘roots and tubers’ exhibited weak cytotoxic activity. The hexane extract (IC 50 = 65.0 µg/mL) from the ‘stems and leaves’ exhibited weaker cytotoxic activity than the chloroform extract (IC 50 = 8.0 µg/mL). Although the juice extract from the ‘roots and tubers’ is frequently consumed for cancer treatment, it exhibited poor cytotoxic activity. Further analysis using an amino acid analyser revealed that the juice extract contained a high concentration of arginine (0.874%). A high tryptophan content (0.800%) was confirmed by NMR and HPLC analysis. Copyright © 2001 John Wiley & Sons, Ltd.