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Anti‐gastric cancer activity and mechanism of natural compound “Heilaohulignan C” isolated from Kadsura coccinea
Author(s) -
Daniyal Muhammad,
Liu Yongbei,
Yang Yupei,
Xiao Feng,
Fan Jialong,
Yu Huanghe,
Qiu Yixing,
Liu Bin,
Wang Wei,
Yuhui Qin
Publication year - 2021
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.7114
Subject(s) - propidium iodide , apoptosis , flow cytometry , cytotoxicity , mtt assay , western blot , microbiology and biotechnology , biology , cancer cell , cancer , chemistry , programmed cell death , cancer research , in vitro , biochemistry , genetics , gene
In this research, we analyzed the antitumor activity of one new compound Heilaohulignan C (B‐6) on the human gastric carcinoma cells. MTT, cell migration, Calcein AM/Propidium Iodide (PI), and flow cytometry in BGC‐823 cell line (gastric tumor). Western blot was utilized to distinguish the protein level. Xenografts nude mice were used for in vivo anticancer analysis. H&E staining and laboratory investigation was accomplished for toxicity study. MTT test demonstrated the cytotoxicity of BGC‐823 cells, Calcein AM/Propidium Iodide (PI) examine indicated increment dead cells proportion with a high dose of B‐6, Flow cytometry (FACS) measure showed that B‐6 influenced gastric cancer cells by initiating apoptosis. Western blot analysis confirmed that (B‐6) decrease the level of Bcl‐2 and increase the level of p53, Bax, and cleaved Caspase‐3, this confirms that the B‐6 doing the apoptosis through caspase and cytochrome C apoptotic pathways. Also, B‐6 particularly decline the tumor volume and tumor size in the xenograft mice. H&E staining additionally supports that B‐6 does not have any toxic impact on the normal tissues. This research supports that B‐6 have pharmacological activity against gastric cancer, by p53 and mitochondrial dependent apoptotic pathway, and have no toxicity on normal tissues.