Premium
Protective effects of irigenin against 1‐methyl‐4‐phenylpyridinium ‐induced neurotoxicity through regulating the Keap1/Nrf2 pathway
Author(s) -
Guo Fen,
Wang Xiaoxue,
Liu Xinxin
Publication year - 2021
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.6926
Subject(s) - neurotoxicity , chemistry , superoxide dismutase , oxidative stress , malondialdehyde , apoptosis , viability assay , nitric oxide , mtt assay , catalase , keap1 , pharmacology , biochemistry , microbiology and biotechnology , biology , toxicity , organic chemistry , transcription factor , gene
The rhizome of Belamcanda chinensis possesses antiinflammatory and antioxidant activities. However, the effect of irigenin, isolated from the rhizome of B. chinensis , on 1‐methyl‐4‐phenylpyridinium (MPP + )‐induced neurotoxicity is unknown. MTT assay showed that MPP + exposure dose dependently inhibited the viability of mouse microglia BV‐2 cells, whereas irigenin suppressed MPP + ‐induced viability reduction. The production of nitric oxide, prostaglandin E2, tumor necrosis factor‐α and interleukin‐6 were increased by MPP + treatment, which were abolished by irigenin treatment. Irigenin‐attenuated MPP + ‐induced increase of malondialdehyde content and activities of superoxide dismutase, catalase and glutathione peroxidase in BV‐2 cells. Irigenin treatment also repressed apoptosis, caspase‐3/7 activity and Cytochrome C expression in MPP + ‐challenged BV‐2 cells. Interestingly, irigenin activated the Keap1/Nrf2 pathway in MPP + ‐induced BV‐2 cells. Nrf2 knockdown attenuated the effects of irigenin on MPP + ‐induced viability reduction, inflammation, oxidative stress and apoptosis in BV‐2 cells. In conclusion, irigenin alleviated MPP + ‐induced neurotoxicity in BV‐2 cells through regulating the Keap1/Nrf2 pathway.