Protective effects of irigenin against  1‐methyl‐4‐phenylpyridinium ‐induced neurotoxicity through regulating the Keap1/Nrf2 pathway | Zendy

Guo Fen | Zendy; Wang Xiaoxue | Zendy; Liu Xinxin | Zendy

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Protective effects of irigenin against 1‐methyl‐4‐phenylpyridinium ‐induced neurotoxicity through regulating the Keap1/Nrf2 pathway

Author(s) -

Guo Fen,

Wang Xiaoxue,

Liu Xinxin

Publication year - 2021

Publication title -

phytotherapy research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 129

eISSN - 1099-1573

pISSN - 0951-418X

DOI - 10.1002/ptr.6926

Subject(s) - neurotoxicity , chemistry , superoxide dismutase , oxidative stress , malondialdehyde , apoptosis , viability assay , nitric oxide , mtt assay , catalase , keap1 , pharmacology , biochemistry , microbiology and biotechnology , biology , toxicity , organic chemistry , transcription factor , gene

The rhizome of Belamcanda chinensis possesses antiinflammatory and antioxidant activities. However, the effect of irigenin, isolated from the rhizome of B. chinensis , on 1‐methyl‐4‐phenylpyridinium (MPP + )‐induced neurotoxicity is unknown. MTT assay showed that MPP + exposure dose dependently inhibited the viability of mouse microglia BV‐2 cells, whereas irigenin suppressed MPP + ‐induced viability reduction. The production of nitric oxide, prostaglandin E2, tumor necrosis factor‐α and interleukin‐6 were increased by MPP + treatment, which were abolished by irigenin treatment. Irigenin‐attenuated MPP + ‐induced increase of malondialdehyde content and activities of superoxide dismutase, catalase and glutathione peroxidase in BV‐2 cells. Irigenin treatment also repressed apoptosis, caspase‐3/7 activity and Cytochrome C expression in MPP + ‐challenged BV‐2 cells. Interestingly, irigenin activated the Keap1/Nrf2 pathway in MPP + ‐induced BV‐2 cells. Nrf2 knockdown attenuated the effects of irigenin on MPP + ‐induced viability reduction, inflammation, oxidative stress and apoptosis in BV‐2 cells. In conclusion, irigenin alleviated MPP + ‐induced neurotoxicity in BV‐2 cells through regulating the Keap1/Nrf2 pathway.

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