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Estrogenic properties of Prunus cerasoides extract and its constituents in MCF‐7 cell and evaluation in estrogen‐deprived rodent models
Author(s) -
Kim SoDam,
Kim Yeojin,
Kim Mingee,
Jeong Hyesoo,
Choi Sang Ho,
Ryu Hyung Won,
Oh SeiRyang,
Lee Sang Woo,
Li Wan Yi,
Wu HongHua,
Zhu Yan,
Wang Xiaoying,
Chang Minsun,
Song Yun Seon
Publication year - 2020
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.6604
Subject(s) - phytoestrogens , estrogen , isoflavones , ovariectomized rat , estrogen receptor , endocrinology , in vivo , medicine , flavones , pharmacology , chemistry , biology , cancer , breast cancer , microbiology and biotechnology , chromatography
Prunus cerasoides (PC) products contain relatively high levels of flavones and isoflavones and may be potential sources of phytoestrogens for postmenopausal symptom relief. We assessed the PC extract (PCE) and its representative constituents in vitro with assays for estrogen receptor alpha binding, estrogen response element transcriptional activity, cell proliferation, and gene expression changes for pS2 in MCF‐7 cells. PCE and its compounds showed strong estrogen receptor binding affinities and estrogen response element induction. A previously undescribed compound (designated as compound 18), now identified as being gentisic acid, 5‐ O ‐β‐D‐(6’‐ O ‐trans‐4‐coumaroyl)‐glucopyranoside, also showed potent estrogenic properties and induced proliferation of MCF‐7 cells. PCE was evaluated for its in vivo uterotrophic effects in immature female rats as well as for its lipid lowering effects in estrogen‐deprived animals. For ovariectomized rats and aged female mice, PCE‐treated groups had lower plasma triglyceride levels compared with control and, for the same comparison, had reduced serum levels of liver stress/damage markers. Our results point to strong estrogenic activities and beneficial metabolic effects for PCE, with properties that put PC and its extracts as promising sources of phytoestrogens for symptom relief in menopausal and postmenopausal cases.