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Antitumour effects of selected plant polyphenols, gallic acid and ellagic acid, on sensitive and multidrug‐resistant leukaemia HL60 cells
Author(s) -
Maruszewska Agnieszka,
Tarasiuk Jolanta
Publication year - 2019
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.6317
Subject(s) - hl60 , gallic acid , dna fragmentation , apoptosis , ellagic acid , programmed cell death , caspase , biology , cytotoxicity , biochemistry , cell culture , reactive oxygen species , chemistry , microbiology and biotechnology , in vitro , antioxidant , polyphenol , genetics
The aim of this study was to examine the antitumour effects of plant phenolic acids, gallic acid (GA) and ellagic acid (EA), on human promyelocytic leukaemia sensitive HL60 cell line and its resistant sublines exhibiting two MDR phenotypes: HL60/VINC (overexpressing P‐glycoprotein) and HL60/MX2 (characterized by the presence of mutated α isoform of topoisomerase II). Both studied compounds exerted comparable cytotoxic activities towards sensitive HL60 cells and their MDR counterparts. It was also found that GA and EA modulated the cellular level of reactive oxygen species in a dose‐dependent and time‐dependent manner. Furthermore, it was demonstrated that GA (IC 90 ) and EA (IC 50 and IC 90 ) significantly increased the percentage of sub‐G1 subpopulation of all studied leukaemia cells causing oligonucleosomal DNA fragmentation. Both compounds used at IC 90 triggered mainly the apoptotic death of these cells. However, GA had no effect on the activity of caspase‐3 as well as caspase‐8 in sensitive HL60 cells and their MDR counterparts. In contrast, EA provoked a significant activation of these caspases in all studied leukaemia cells. It was also found that lysosomes were not involved in triggering programmed death of sensitive HL60 and MDR cells by GA and EA.