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Hepatoprotective activity of praecoxin A isolated from Melaleuca ericifolia against carbon tetrachloride‐induced hepatotoxicity in mice. Impact on oxidative stress, inflammation, and apoptosis
Author(s) -
AlSayed Eman,
AbdelDaim Mohamed M.,
Khattab Mohamed Abdelrazik
Publication year - 2019
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.6242
Subject(s) - oxidative stress , carbon tetrachloride , lipid peroxidation , pharmacology , hepatoprotection , ccl4 , antioxidant , glutathione , toxicity , chemistry , bilirubin , necrosis , inflammation , medicine , biochemistry , immunology , organic chemistry , enzyme
The hepatoprotective activity of praecoxin A, an ellagitannin from Melaleuca ericifolia , was determined against CCl 4 ‐induced toxicity in mice. Praecoxin A was administered (25, 50, and 100 mg/kg) for 5 days followed by CCl 4 . Praecoxin A markedly ameliorated the CCl 4 ‐induced increase in AST (by 19, 52, and 56%), ALP (22, 45, and 48%), ALT (11, 47, and 54%), total bilirubin (14, 27, and 28%), and MDA (26, 44, and 51%) at the tested doses, respectively, as compared with CCl 4 group. It was evident that praecoxin A significantly ( p < 0.001) increased the antioxidant parameters GSH (45, 99, and 137%) and SOD (61, 129, and 159%). Histological findings revealed a marked amelioration of hepatocyte degeneration, necrosis, inflammatory cell infiltration, and hemorrhage in the groups treated with praecoxin A. COX‐2 and caspase‐3 hepatic expressions were significantly downregulated ( p < 0.001) in praecoxin A‐treated groups (up to 57, 83, and 93% for COX‐2 and by 30, 82, and 99% for caspase‐3). These findings suggest that praecoxin A exerts a beneficial effect against oxidative stress by reducing lipid peroxidation, enhancing the antioxidant defense status, and protecting against the histopathological changes induced by CCl 4 . This study highlights a promising natural hepatoprotective candidate derived from M. ericifolia that might be an alternative to silymarin.