Premium
Effects of oligomeric grape seed proanthocyanidins on L‐ NAME ‐ induced hypertension in pregnant mice: Role of oxidative stress and endothelial dysfunction
Author(s) -
Zhu Fengqin,
Hu Juan,
Lv Fahui,
Cheng Pan,
Gao Shan
Publication year - 2018
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.6119
Subject(s) - oxidative stress , endothelial dysfunction , creatinine , nitric oxide , endocrinology , medicine , blood urea nitrogen , kidney , aorta , blood pressure , grape seed , pharmacology , chemistry , food science
The aim of this study was to investigate the effects of Grape Seed Proanthocyanidins (GSP) on Nω‐Nitro‐L‐arginine methyl ester‐induced hypertension in pregnant mice. Fifty Kunming mice were randomized into control, control + GSP, model, and model + GSP. Three weeks later, the artery systolic blood pressure was examined and the related pathological changes were detected. Aorta relaxation function was assessed by aorta ring apparatus. Blood urea nitrogen and serum creatinine were measured by an automatic biochemistry analyzer. Colorimetric analysis, enzyme‐linked immunosorbent assay, immunofluorescence, and western blot were applied to detect related indicator in serum, cardiac, and kidney tissues. The results showed that GSP treatment for 3 weeks could improve cardiovascular and kidney remodeling indexes and decrease blood urea nitrogen and serum creatinine content in serum, as well as could ameliorate oxidative stress status and endothelial dysfunction. Therefore, it is for the first time found that GSP exerts protective effect against Nω‐Nitro‐L‐arginine methyl ester‐induced hypertension in pregnant mice, which provided a theoretical basis for potential application in the clinic.