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Radioprotective effects of dammarane sapogenins against 60 Co‐induced myelosuppression in mice
Author(s) -
Dong Liming,
Yang Yanyan,
Lu Yan,
Lu Cong,
Lv Jingwei,
Jiang Ning,
Xu Qiuxia,
Gao Yue,
Chang Qi,
Liu Xinmin
Publication year - 2018
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.6027
Subject(s) - haematopoiesis , bone marrow , peripheral blood cell , progenitor cell , blood cell , spleen , white blood cell , stem cell , granulocyte , sapogenin , biology , chemistry , immunology , pathology , medicine , microbiology and biotechnology , alternative medicine
Radiotherapy frequently induces failure of hematopoietic system and leads to myelosuppression. The objective of this study was to investigate the protective effect of dammarane sapogenins (DS), the hydrolysed product of the constituent ginsenosides of Panax ginseng , which are produced by gut metabolism, on radiation‐induced hematopoietic injury. Mice were exposed to 3.5 Gy 60 Co γ‐rays of total body radiation at a dose rate of 1.60 Gy per minute and treated with DS or granulocyte colony‐stimulating factor immediately after radiation. The general condition of the mice, the peripheral blood cell counts, multiple colony forming unit (CFU) assays of hematopoietic progenitor cells, hematopoietic stem cell counts, bone marrow histology, and spleen colony forming unit counts were then investigated. Our results indicated that administration with DS could ameliorate 60 Co‐irradiation induced damage and significantly increase the number of peripheral blood cells (white blood cells and platelets), 5 types of hematopoietic progenitor cells CFU (CFU‐GM, CFU‐E, BFU‐E, CFU‐Meg, and CFU‐GEMM), hematopoietic stem cell (Lin − c‐kit + Scal‐1 + ) numbers, and CFUs in the spleen, as well as improved bone marrow histopathology. All together, these results confirmed the enhancement of DS on hematopoiesis.