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Hypolipidemic activity and mechanisms of the total phenylpropanoid glycosides from Ligustrum robustum (Roxb.) Blume by AMPK‐SREBP‐1c pathway in hamsters fed a high‐fat diet
Author(s) -
Yang Runmei,
Chu Xinxin,
Sun Le,
Kang Zhuoying,
Ji Min,
Yu Ying,
Liu Ying,
He Zhendan,
Gao Nannan
Publication year - 2018
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.6023
Subject(s) - fatty acid synthase , biochemistry , sterol o acyltransferase , chemistry , cholesterol , acyltransferase , phenylpropanoid , protein kinase a , sterol regulatory element binding protein , sterol , endocrinology , medicine , biology , lipoprotein , fatty acid , enzyme , biosynthesis
The aim of this study was to evaluate the hypolipidemic effect and mechanisms of total phenylpropanoid glycosides extracted from Ligustrum robustum (Roxb.) Blume (LRTPG) in hamsters fed a high‐fat diet and to discover bioactive components in HepG2 cell model induced by oleic acid. LRTPG of high (1.2 g/kg), medium (0.6 g/kg), and low (0.3 g/kg) doses was administrated daily for 21 consecutive days in hamsters. We found that in hamsters fed a high‐fat diet, LRTPG effectively reduced the concentrations of plasma triglycerides (TG), free fatty acid, total cholesterol, low‐density lipoprotein cholesterol, and hepatic TG and total cholesterol. And the compounds acteoside, ligupurpuroside A, ligupurpuroside C, and ligupurpuroside D significantly inhibited lipid accumulation in HepG2 cell at the concentration of 50 μmol/L. Mechanism research demonstrated that LRTPG increased the levels of phospho–AMP‐activated protein kinase and phospho‐sterol regulatory element binding protein‐1c in liver, further to suppress the downstream lipogenic genes as stearoyl‐CoA desaturase 1, glycerol‐3‐phosphate acyltransferase, 1‐acylglycerol‐3‐phosphate O‐acyltransferase 2, and diacylglycerol acyltransferase 2. In addition, LRTPG increased the hydrolysis of circulating TG by up‐regulating lipoprotein lipase activities. These results indicate that LRTPG prevents hyperlipidemia via activation of hepatic AMP‐activated protein kinase‐sterol regulatory element binding protein‐1c pathway.