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Genipin ameliorates diet‐induced obesity via promoting lipid mobilization and browning of white adipose tissue in rats
Author(s) -
Guan Lili,
Gong Dezheng,
Yang Sirao,
Shen Nana,
Zhang Sai,
Li Yuchen,
Wu Qiong,
Yuan Bo,
Sun Yiping,
Dai Ning,
Zhu Liang,
Zou Yuan
Publication year - 2018
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.6022
Subject(s) - genipin , endocrinology , medicine , white adipose tissue , lipolysis , adipose triglyceride lipase , adipose tissue , insulin resistance , adipocyte , hormone sensitive lipase , lipogenesis , lipid metabolism , diet induced obese , chemistry , biology , insulin , biochemistry , chitosan
Genipin is the major active component of Gardeniae fructus and has been shown to ameliorate diabetes and insulin resistance in rat models. In this study, we first investigated the effect of genipin on obesity and the related lipid metabolism mechanisms in diet‐induced obese rats. Our results showed that genipin reduced body weight, food intake, and visceral fat mass; ameliorated dyslipidemia, glucose intolerance, insulin intolerance, adipocyte hypertrophy, and hepatic steatosis; and reduced serum tumor necrosis factor‐α level in diet‐induced obese rats. Quantitative real‐time reverse‐transcription polymerase chain reaction results further illustrated that genipin promoted lipolysis and β‐oxidation of fatty acid by upregulating gene expressions of hormone‐sensitive lipase and adipose triglyceride lipase in white adipose tissue (WAT) and peroxisome proliferator‐activated receptor‐α and carnitine palmitoyltransferase 1α in hepatic tissue. Moreover, genipin promoted browning of WAT by upregulating the mRNA and protein levels of uncoupling protein 1 and PRD1‐BF1‐RIZ1 homologous domain containing 16 in WAT. Additionally, genipin inhibited gene expressions of activin receptor‐like kinase 7, tumor necrosis factor‐α, and interlukin‐6 in WAT. These results indicated that genipin had a potential therapeutic role in obesity, in which regulation of lipid mobilization and browning of WAT were involved.