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A pilot pharmacokinetic study of miroestrol and deoxymiroestrol on rabbit sera using polyclonal antibody‐based icELISA analysis
Author(s) -
Kitisripanya Tharita,
Udomsin Orapin,
Komaikul Jukrapun,
Inyai Chadathorn,
Limsuwanchote Supattra,
Yusakul Gorawit,
Putalun Waraporn
Publication year - 2018
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5991
Subject(s) - polyclonal antibodies , pharmacokinetics , rabbit (cipher) , pharmacology , antibody , medicine , lagomorpha , chromatography , traditional medicine , chemistry , immunology , mathematics , statistics
Miroestrol (ME) and deoxymiroestrol (DME) are the most potent phytoestrogens and bioactive markers in Pueraria candollei var. mirifica tuberous roots. To understand their pharmacokinetic profiles, a pharmacokinetic study of ME and DME, at 0.43 and 0.21 mg per kg body weight, respectively, in three rabbits was performed after orally administering a single dose of P. candollei var. mirifica enriched fraction extract. Two established polyclonal antibody‐based indirect competitive enzyme‐linked immunosorbent assays were validated to determine ME and DME in rabbit sera. In rabbits, the area under the 0‐ to 48‐hr concentration‐time curve of ME and DME were 854.92 and 1,692.84 ng·h/ml, respectively. The maximum concentration of ME was measured 1 hr after administration as 69.62 ± 8.28 ng/ml, and the maximum concentration of DME was measured at 3 hr as 81.8 ± 5.43 ng/ml. These results provide an initial approach for designing and studying the relationship between the ME and DME levels and their therapeutic effects based on their pharmacokinetic profiles.