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Pycnogenol Cytotoxicity in Pancreatic INS‐1E β cells Induced by Calcium Dysregulation
Author(s) -
Viskupicova Jana,
Zizkova Petronela,
Rackova Lucia,
Horakova Lubica
Publication year - 2017
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5894
Subject(s) - calcium in biology , calcium , intracellular , apoptosis , skeletal muscle , cytotoxicity , endoplasmic reticulum , chemistry , flavonoid , calcium metabolism , viability assay , pharmacology , microbiology and biotechnology , medicine , endocrinology , biology , biochemistry , in vitro , antioxidant
Natural standardized flavonoid extract from the bark of Pinus pinaster , Pycnogenol (Pyc), was recently found to decrease intensively the activity of sarcoplasmic reticulum Ca 2+ ‐ATPase of rabbit skeletal muscle (SERCA1). On the basis of this inhibitory effect in a cell‐free system and similarities of SERCA1 to its other isoforms, proapoptotic properties of Pyc may be expected in cellular systems. Pycnogenol (40–100 μg/mL) induced a concentration‐dependent decrease of the viability of pancreatic INS‐1E β cells associated with induction of apoptosis. In addition, intracellular Ca 2+ level increase was found along with reduction of protein expression level of SERCA2b and impairment of insulin secretion by β cells. These facts indicate that Pyc may induce apoptosis by impairment of calcium homeostasis. Copyright © 2017 John Wiley & Sons, Ltd.