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The Potential Impact of Radix Paeoniae Alba in Embryonic Development of Mice
Author(s) -
Xu Wei,
Xu Ling,
Deng Bin,
Leng Jing,
Tang g,
Zhao Li Chun,
Zhou Hong Hai,
Zhao Zhong Zhen,
Yang Zhi Jun,
Xiao Ting Ting,
Tian Xiao Ying,
Ho Alan H.M.,
Chan Nickie W.K.,
Chow Yeuk Lung,
Chow Chi Yi,
Xu Min
Publication year - 2017
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5864
Subject(s) - fetus , dose , embryonic stem cell , andrology , cytotoxicity , medicine , gestation , apoptosis , pharmacology , significant difference , radix (gastropod) , embryogenesis , immunology , traditional medicine , pregnancy , biology , in vitro , embryo , biochemistry , botany , microbiology and biotechnology , genetics , gene
Although Radix Paeoniae Alba (RPA) has been ranked as one of the top 6 herbs used frequently to prevent and treat miscarriages clinically, there is no clear evidence regarding its safety in embryonic development. This study aims to evaluate the potential impacts of RPA on embryonic stem cells (ESCs) and pregnant mice. Cytotoxicity assays of the extract were performed in ESCs and 3T3 cells. Pregnant ICR mice were orally treated with RPA extracts at dosages of 0 (G1 group as negative controls), 2, 8 and 32 g/kg/day (G2, G3 and G4 groups) respectively from the gestation day (Gd) 6–15. On Gd 18, there was no significant difference in the IC 50 values between ESCs and 3T3 cells ( p > 0.05). There was no significant difference in the maternal and fetal evaluations among four groups ( p > 0.05). Fetal IL‐2, IL‐2r, TNF‐α, TNF‐αr, IL‐4, IL‐4r, IL‐10r, IL‐17 and IL‐17r of G4 group were significantly lower than G1 group ( p < 0.05). In conclusion, RPA at dosage of 32 g/kg/day (16‐folds of human daily dosage) did not cause adverse impact in cultured ESCs and pregnant mice. RPA might down‐regulate fetal Th1/Th2/Th17 cytokines and receptors maybe beneficial to embryonic survival and development. Copyright © 2017 John Wiley & Sons, Ltd.