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The In Vitro and In Vivo Biological Activities of the Leaf of Cape Myrtle, Myrsine africana L.
Author(s) -
Gul Hina,
Ahmad Mushtaq,
Zafar Muhammad,
Sheeraz Ahmad Muhammad,
Abid Ambreen,
Hira Syeda,
Shah Imam,
Gulfraz Muhammad
Publication year - 2017
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5853
Subject(s) - in vivo , antioxidant , quercetin , traditional medicine , chemistry , in vitro , alkaline phosphatase , polyphenol , dpph , biochemistry , biology , enzyme , medicine , microbiology and biotechnology
The cape myrtle, Myrsine africana L., is a widely used medicinal plant, which has not been well investigated. We assessed the in vivo hepatoprotective and in vitro antiproliferative and antioxidant effects of leaf extracts of M. africana chemically profiled using high‐performance liquid chromatography. Three flavonoids were quantified, and gas chromatography–mass spectrometry analysis revealed the presence of common fatty acids. The animal study was conducted on mice treated with CCl4, using three doses each of the methanol and chloroform extract (100, 200 and 300 mg/kg b.w.),with silymarin as a positive control. Hepatoprotective effects were determined by analyzing blood for liver marker and antioxidant enzymes, direct bilirubins and total proteins. The methanol extract (300 mg/kg b.w.) showed the strongest hepatoprotective effects against abnormalities produced by CCl4. The in vivo hepatoprotective effects correlated well with the in vitro antioxidant and antiproliferative activities and with high levels of flavonoids in the extracts. Finally, molecular docking studies of the constituent quercetin were undertaken in silico and several sites of binding to human estrogen receptor (ER) protein, linked with alkaline phosphatase, identified. Copyright © 2017 John Wiley & Sons, Ltd.