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Effect of Perilla frutescens Extracts on Porcine Jejunal Epithelial Cells
Author(s) -
Kaufmann Christine M.,
Letzel Thomas,
Grassmann Johanna,
Pfaffl Michael W.
Publication year - 2017
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5750
Subject(s) - perilla frutescens , perilla , downregulation and upregulation , cell culture , cell growth , cell cycle , cell , biology , cyclin d1 , apoptosis , gene expression , microbiology and biotechnology , biochemistry , gene , raw material , ecology , genetics
Green‐leaved Perilla frutescens extracts were investigated on their effect on cell proliferation of the porcine jejunal epithelial cell line, IPEC‐J2, as well as on the gene expression of cell cycle or cancer‐related genes. Some extracted compounds were, however, susceptible to degradation in cell culture medium, whereas others were found to be stable during the entire experimental time. Control experiments also included the assessment of H 2 O 2 generation in cell culture medium caused by oxidation of natural extract compounds, which was proved to be absent at low extract concentrations. A fast and significant inhibition of cell growth at low physiological extract concentrations could be observed. This finding, along with an immediate downregulation of 67 kDa laminin receptor and cyclin D1 expression, can be accounted to the presence of Perilla frutescens extract. Copyright © 2016 John Wiley & Sons, Ltd.