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The Water Fraction of Calendula officinalis Hydroethanol Extract Stimulates In Vitro and In Vivo Proliferation of Dermal Fibroblasts in Wound Healing
Author(s) -
Dinda Manikarna,
Mazumdar Swagata,
Das Saurabh,
Ganguly Durba,
Dasgupta Uma B,
Dutta Ananya,
Jana Kuladip,
Karmakar Parimal
Publication year - 2016
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5678
Subject(s) - ctgf , wound healing , in vivo , calendula officinalis , pharmacology , rutin , granulation tissue , chemistry , biology , traditional medicine , growth factor , medicine , biochemistry , immunology , antioxidant , receptor , microbiology and biotechnology
The active fraction and/or compounds of Calendula officinalis responsible for wound healing are not known yet. In this work we studied the molecular target of C. officinalis hydroethanol extract (CEE) and its active fraction (water fraction of hydroethanol extract, WCEE) on primary human dermal fibroblasts (HDF). In vivo , CEE or WCEE were topically applied on excisional wounds of BALB/c mice and the rate of wound contraction and immunohistological studies were carried out. We found that CEE and only its WCEE significantly stimulated the proliferation as well as the migration of HDF cells. Also they up‐regulate the expression of connective tissue growth factor (CTGF) and α‐smooth muscle actin (α‐SMA) in vitro . In vivo , CEE or WCEE treated mice groups showed faster wound healing and increased expression of CTGF and α‐SMA compared to placebo control group. The increased expression of both the proteins during granulation phase of wound repair demonstrated the potential role of C. officinalis in wound healing. In addition, HPLC‐ESI MS analysis of the active water fraction revealed the presence of two major compounds, rutin and quercetin‐3‐ O ‐glucoside. Thus, our results showed that C. officinalis potentiated wound healing by stimulating the expression of CTGF and α‐SMA and further we identified active compounds. Copyright © 2016 John Wiley & Sons, Ltd.