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Antenatal Saireito (TJ‐114) Can Improve Pulmonary Hypoplasia and Pulmonary Vascular Remodeling in Nitrofen‐Induced Congenital Diaphragmatic Hernia
Author(s) -
Hirako Shima,
Tsuda Hiroyuki,
Kotani Tomomi,
Sumigama Seiji,
Mano Yukio,
Nakano Tomoko,
Imai Kenji,
Li Hua,
Toyokuni Shinya,
Kikkawa Fumitaka
Publication year - 2016
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5645
Subject(s) - congenital diaphragmatic hernia , nitrofen , pulmonary hypoplasia , medicine , diaphragmatic hernia , hypoplasia , diaphragmatic breathing , pulmonary hypertension , cardiology , pulmonary vasculature , fetus , anatomy , hernia , surgery , pathology , pregnancy , biology , genetics , alternative medicine
Congenital diaphragmatic hernia (CDH) can induce lung hypoplasia and pulmonary hypertension and is associated with high mortality. The purpose of this study is to examine the efficacy and safety of antenatal Saireito (TJ‐114), a traditional Japanese herbal medicine, in a rat CDH model. Sprague‐Dawley rats were exposed to an herbicide (nitrofen, 100 mg) on embryonic day 9 (E9) to induce CDH, and antenatal Saireito (2000 mg/kg/day) was orally administered from E10 to E20. On E21, fetuses were delivered. Antenatal Saireito significantly decreased the incidence of CDH ( p < 0.01), increased lung volume ( p < 0.01), improved alveolarization and pulmonary artery remodeling using histological analysis, and improved respiratory function using gasometric analysis (pH; p < 0.05, and PCO 2 ; p < 0.01). In addition, antenatal Saireito significantly decreased endothelin‐1 and endothelin receptor A expression in the pulmonary arteries. Taken together, our results demonstrated that antenatal Saireito can improve fetal pulmonary hypoplasia and pulmonary vascular remodeling and, as a result, can improve respiratory function in a rat CDH model. Copyright © 2016 John Wiley & Sons, Ltd.