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Acetonic Extract from the Feijoa sellowiana Berg. Fruit Exerts Antioxidant Properties and Modulates Disaccharidases Activities in Human Intestinal Epithelial Cells
Author(s) -
Turco Fabio,
Palumbo Ilaria,
Andreozzi Paolo,
Sarnelli Giovanni,
De Ruberto Francesca,
Esposito Giuseppe,
Basile Adriana,
Cuomo Rosario
Publication year - 2016
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5629
Subject(s) - sucrase , disaccharidase , lipid peroxidation , viability assay , antioxidant , lactase , biochemistry , biology , cell growth , endocrinology , medicine , chemistry , in vitro , enzyme
Feijoa sellowiana fruit has been shown to possess various biological activities, such as anti‐bacterial and anti‐cancer properties, in a variety of cellular models, but its activity on human intestinal epithelial cells has never been tested. The purpose of this study was to investigate the effects of the acetonic extract of F. sellowiana fruits on the viability, membrane peroxidation, disaccharidases activities and proliferation of in vitro models of human intestinal epithelial cells. To obtain this goal, Caco‐2 and HT‐29 cells were exposed to the acetonic extract for 24 h. Cell proliferation, viability, lactase and sucrase‐isomaltase activity and H 2 O 2 ‐induced membrane lipid peroxidation were tested. We found that, compared to control conditions, the acetonic extract significantly increased lactase and sucrase‐isomaltase activity in Caco‐2, but not HT‐29, cells, decreased proliferation, had no effects on viability and restored lipid peroxidation in both cell models. This study suggests that the acetonic extract improves lactase and sucrase‐isomaltase activity, inhibits cell proliferation, have no cytotoxic effects and prevent lipid peroxidation of intestinal epithelial cells. These effects may be exploited in case of disaccharidases deficit and also as an adjuvant treatment of diseases related to oxidative stress. Copyright © 2016 John Wiley & Sons, Ltd.