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Resveratrol Pretreatment Affects CYP2E1 Activity of Chlorzoxazone in Healthy Human Volunteers
Author(s) -
Bedada Satish Kumar,
Neerati Prasad
Publication year - 2016
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5549
Subject(s) - chlorzoxazone , cmax , pharmacokinetics , pharmacology , cyp2e1 , chemistry , metabolite , elimination rate constant , area under the curve , oral administration , volume of distribution , medicine , enzyme , biochemistry , cytochrome p450
The purpose of the present study was to investigate the effect of resveratrol (RSV) pretreatment on CYP2E1 enzyme activity and pharmacokinetics of chlorzoxazone (CHZ) in healthy human volunteers. The open‐label, two period, sequential study was conducted in 12 healthy human volunteers. A single dose of RSV 500 mg was administered once daily for 10 days during treatment phase. A single dose of CHZ 250 mg was administered during control and after treatment phases under fasting conditions. The blood samples were collected after CHZ dosing at predetermined time intervals and analyzed by HPLC. RSV pretreatment significantly enhanced the maximum plasma concentration (C max ), area under the curve (AUC) and half life (T 1/2 ) and significantly decreased elimination rate constant (K el ), apparent oral clearance (CL/F) and apparent volume of distribution (Vd/F) of CHZ as compared to that of control. In addition, RSV pretreatment significantly decreased the metabolite to parent (6‐OHCHZ/CHZ) ratios of C max , AUC and T 1/2 and significantly increased the K el ratio of 6‐OHCHZ/CHZ, which indicated the reduced formation of CHZ to 6‐OHCHZ. The results suggest that the altered CYP2E1 enzyme activity and pharmacokinetics of CHZ might be attributed to RSV mediated inhibition of CYP2E1 enzyme. Thus, there is a potential pharmacokinetic interaction between RSV and CHZ. The inhibition of CYP2E1 by RSV may provide a novel approach for minimizing the hepatotoxicity of ethanol. Copyright © 2015 John Wiley & Sons, Ltd.