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Hedera nepalensis K. Koch: A Novel Source of Natural Cancer Chemopreventive and Anticancerous Compounds
Author(s) -
Jafri Laila,
Saleem Samreen,
Kondrytuk Tamara P.,
Haq Ihsanul,
Ullah Nazif,
Pezzuto John M.,
Mirza Bushra
Publication year - 2016
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5546
Subject(s) - lupeol , hela , traditional medicine , sulforhodamine b , cancer cell , chemistry , ethyl acetate , du145 , ic50 , mtt assay , biology , cytotoxic t cell , cancer , biochemistry , lncap , in vitro , medicine , genetics
Traditional medicinal plants are often used for both the prevention and the treatment of local diseases. Taking into consideration the medicinal importance of Hedera nepalensis within local Pakistani traditions, the present study was undertaken to analyze the in vitro cancer chemopreventive and cytotoxic properties of the plant. The in vitro cancer chemopreventive testing was performed using nitrite assay, NFκB assay, aromatase assay, and quinone reductase 1 (QR1) assay. The cytotoxic potential was evaluated on three cancer‐cell lines: MCF‐7, MDA‐MB‐231, and HeLa using sulforhodamine B (SRB) assay. The results of cancer chemopreventive assays show that n‐ hexane and ethyl acetate fractions of tested plant have promising cancer chemopreventive potential. Lupeol isolated from n ‐hexane as well as ethyl acetate fraction showed lowest IC 50 (0.20 ± 1.9 μM) in NFκB assay. Crude extract and its fractions inhibited the growth of three cancer cell lines by more than 60%, IC 50 value of lupeol varied from 2.32 to 10.2 μM. HPLC‐DAD‐based quantification of lupeol in different plant tissues demonstrated that leaves of H. nepalensis are a rich source of lupeol (0.196 mg/100 mg dry weight). Our data have shown that H. nepalensis harbors cancer chemopreventive and cytotoxic agents. Copyright © 2015 John Wiley & Sons, Ltd.