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Antifungal Saponins from the Maya Medicinal Plant Cestrum schlechtendahlii G. Don (Solanaceae)
Author(s) -
Ta Chieu Anh Kim,
GuerreroAnalco J. Antonio,
Roberts Elizabeth,
Liu Rui,
Mogg Christopher D.,
Saleem Ammar,
OtárolaRojas Marco,
Poveda Luis,
SanchezVindas Pablo,
Cal Victor,
Caal Federico,
Subramaniam Rajagopal,
Smith Myron L.,
Arnason John T.
Publication year - 2016
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5545
Subject(s) - saponin , traditional medicine , antifungal , cryptococcus neoformans , candida albicans , pharmacognosy , botany , solanaceae , minimum inhibitory concentration , biology , chemistry , stereochemistry , biological activity , microbiology and biotechnology , antimicrobial , biochemistry , medicine , in vitro , gene , alternative medicine , pathology
Bioassay‐guided fractionation of the crude extract (80% EtOH) of the leaves of Cestrum schlechtendahlii , a plant used by Q'eqchi' Maya healers for treatment of athlete's foot, resulted in the isolation and identification of two spirostanol saponins (1 and 2). Structure elucidation by MS, 1D‐NMR, and 2D‐NMR spectroscopic methods identified them to be the known saponin (25 R )‐1β,2α‐dihydroxy‐5α‐spirostan‐3‐β‐yl‐ O ‐α‐ l ‐rhamnopyranosyl‐(1 → 2)‐β‐ d ‐galactopyranoside (1) and new saponin (25 R )‐1β,2α‐dihydroxy‐5α‐spirostan‐3‐β‐yl‐ O ‐β‐ d ‐galactopyranoside (2). While 2 showed little or no antifungal activity at the highest concentration tested, 1 inhibited growth of Saccharomyces cerevisiae (minimum inhibitory concentration (MIC) of 15–25 μM), Candida albicans , Cryptococcus neoformans , and Fusarium graminearum (MIC of 132–198 μM). Copyright © 2015 John Wiley & Sons, Ltd.