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Antitumor Activity of Total Flavonoids from Daphne genkwa in Colorectal Cancer
Author(s) -
Du WenJuan,
Yang XiaoLin,
Song ZiJing,
Wang JiaoYing,
Zhang WenJun,
He Xin,
Zhang RunQi,
Zhang ChunFeng,
Li Fei,
Yu ChunHao,
Wang ChongZhi,
Yuan ChunSu
Publication year - 2016
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5540
Subject(s) - colorectal cancer , traditional medicine , medicine , pharmacognosy , thymelaeaceae , cancer , biology , botany , in vitro , biological activity , biochemistry
Daphne genkwa Sieb.et Zucc. is a well‐known medicinal plant. This study was designed to investigate the anticancer effects of total flavonoids in D . genkwa (TFDG) in vitro and in vivo . HT‐29 and SW‐480 human colorectal cancer cells were cultured to investigate the anticancer activity of TFDG. In addition, the Apc Min /+ mouse model was applied in the in vivo experiment. Results of the cell experiment revealed that TFDG possessed significant inhibitory effects on HT‐29 and SW‐480 human colorectal cancer cells (both p < 0.01). Furthermore, our in vivo data showed that after treatment with TFDG, there was a significant increase in life span (both p < 0.01) and tumor numbers were reduced in the colon (both p < 0.01), which was supported by the data of tumor distribution, body weight changes and organ index. Our results also indicated that expressions of interleukin (IL)‐1α, IL‐1β, IL‐6, granulocyte colony‐stimulating factor and granulocyte‐macrophage colony‐stimulating factor in gut tissue were downregulated by treatments of TFDG, and immunity cytokine secretions in the serum were regulated after oral administration of TFDG. Taken together, these findings suggested that TFDG has a potential clinical utility in colorectal cancer therapeutics, and TFDG's action is likely linked to its ability to regulate immune function and inhibit the production of inflammatory cytokines. Copyright © 2015 John Wiley & Sons, Ltd.