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Polyphenolic Secondary Metabolites Synergize the Activity of Commercial Antibiotics against Clinical Isolates of β‐Lactamase‐producing Klebsiella pneumoniae
Author(s) -
Dey Diganta,
Ghosh Subhalakshmi,
Ray Ratnamala,
Hazra Banasri
Publication year - 2016
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.5527
Subject(s) - klebsiella pneumoniae , antibiotics , microbiology and biotechnology , polyphenol , klebsiella , biology , traditional medicine , antibacterial agent , medicine , escherichia coli , biochemistry , gene , antioxidant
Emergence of worldwide antimicrobial resistance prompted us to study the resistance modifying potential of plant‐derived dietary polyphenols, mainly caffeic acid, ellagic acid, epigallocatechin‐3‐gallate (EGCG) and quercetin. These compounds were studied in logical combination with clinically significant antibiotics (ciprofloxacin/gentamicin/tetracycline) against Klebsiella pneumoniae , after conducting phenotypic screening of a large number of clinical isolates and selecting the relevant strains possessing extended‐spectrum β‐lactamase (ESBL) and K . pneumoniae carbapenemase (KPC)‐type carbapenemase enzymes only. The study demonstrated that EGCG and caffeic acid could synergize the activity of tested antibiotics within a major population of β‐lactamase‐producing K . pneumoniae . In spectrofluorimetric assay, ~17‐fold greater ciprofloxacin accumulation was observed within K . pneumoniae cells pre‐treated with EGCG in comparison with the untreated control, indicating its ability to synergize ciprofloxacin to restrain active drug‐efflux. Further, electron micrograph of ESBL‐producing K . pneumoniae clearly demonstrated the prospective efficacy of EGCG towards biofilm degradation. Copyright © 2015 John Wiley & Sons, Ltd.